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Suggested Response - Q1

*Answer all three parts of this question

a)    Give a definition of yeast propagation.

b)    What criteria are used to judge methods used for preserving yeast cultures?

c)    *What is considered, at present, to be the best method of preserving yeast cultures for long term storage?

Response:

a)    Propagation is the periodic introduction of a new yeast culture of guaranteed identity and purity, into the brewery.

b)    Yeast should be stored in a way that ensures it remains pure, does not lose viability and is preserved in a way that does not cause any genetic variants to arise.

c)    The best method currently considered for preserving yeast cultures is storage in liquid nitrogen at -196 ºC.

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