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Self-Assessment Questions

1. ** Explain the differences and connections between obligate and facultative, aerobic and anaerobic, microaerophiles and aerotolerant microbes, and homofermentative and heterofermentative metabolic pathways.

2. ** Discuss how we traditionally isolate contaminating microorganisms within the brewery environment.

3. ** Describe a media and the principles used when selecting for each of the following microorganisms:

a) Lactic acid bacteria (LAB)

b) Enterobacteriaceae

c) Pectinatus

d) Zymomonas

e) Brettanomyces

f) Non-Saccharomyces wild yeast

4. ** Your brewery wishes to reduce the time taken to detect beer spoiling bacteria. Which test would you recommend and why?

5. *** Discuss the factors affecting the ability of bacteria to grow in wort or beer. How do different species exploit the changing processing conditions to survive?

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